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Viral lesion culture
Viral lesion culture


Definition:

Herpes viral culture of a lesion is a laboratory test to check if a skin sample is infected with the herpes simplex virus.



Alternative Names:

Culture - herpes simplex virus; Herpes simplex virus culture



How the test is performed:

A sample from a skin lesion (often a genital sore) or blister is needed. The health care provider will collect the sample when you are having an acute outbreak and place it in a laboratory container. The sample must include cells, not just fluid from the blister, since the virus is in the skin cells of the blister or ulcer.

At the laboratory, the sample is placed in a special dish and watched for the growth of the herpes simplex virus, or substances related to the virus. Special tests may also be done to determine whether it is herpex simplex virus type 1 or 2.

Results are available within 16 hours to 7 days, depending on the laboratory method used.



How to prepare for the test:

The sample must be collected during the worst part of an outbreak. This is considered the acute phase of infection.



How the test will feel:

When the sample is collected, you may feel a scraping or sticky sensation. Sometimes a sample from the throat or eyes is needed. This involves rubbing a sterile swab against eyes or over the throat area.



Why the test is performed:

The test is performed to confirm herpes simplex infection. However, the diagnosis is often made by physical examination, rather than diagnostic testing.



Normal Values:

A normal result means that the herpes simplex virus did not grow in the laboratory dish. This is a sign that the skin sample is free of such infection.



What abnormal results mean:

Abnormal results may mean that you have an active infection with herpes simplex virus. An example of such an infection is herpes genitalis (genital herpes simplex).

Abnormal results may also be due to asymptomatic viral shedding.



What the risks are:

Risks include slight bleeding or infection in the area where the skin sample was removed.



Special considerations:

Viral culture for herpes is a poor test and is often falsely negative (but extremely rarely falsely positive). That is, even with a negative result, the person could still have herpes.

Newer herpes simplex fluorescent antibody testing may be an alternative. Please consult your doctor.




Review Date: 12/3/2007
Reviewed By: D. Scott Smith, M.D., MSc, DTM&H, Chief of Infectious Disease & Geographic Medicine, Kaiser Redwood City, CA & Adjunct Assistant Professor, Stanford University. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.

The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.
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